Method Development for Successful HILIC Separations

Presented by Gemma Lo from Avantor

Gemma is a Global Portfolio Manager at Avantor, based in the UK, responsible for the self-manufactured chromatography portfolio which includes HPLC, UHPLC, GC, TLC and sample preparation columns and workflow solvents. Gemma has over a decade of experience working in the Hichrom technical support team, troubleshooting chromatography methods, recommending suitable column chemistries and supporting the vast range of chromatography columns. Additional experience in HPLC column manufacture and business development are used to support the portfolio. Originally a Biochemist, focus areas include the analysis of biomolecules and polar analytes by various chromatographic techniques and working in close collaboration with the Avantor R&D and applications teams.

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Hydrophilic interaction liquid chromatography (HILIC) separations have gained prominence in many application areas. The ability to retain neutral and ionisable polar species makes HILIC particularly popular for metabolomics and drug development studies and pairs well with mass spec (MS) detection. This seminar is designed to help the new HILIC chromatographer avoid the typical pitfalls that HILIC presents by highlighting the critical parameters involved in HILIC separations. Discussions on column equilibration times and sample diluent challenges are included. The seminar also covers a generally applicable selectivity-based method development platform designed for implementation across HILIC LC and LC-MS platforms.

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